Illumina HiSeq 2000 sequencing; RNA sequencing of beech roots and ass... - SRA

ERX10644821: Illumina HiSeq 2000 sequencing; RNA sequencing of beech roots and associated fungi
4 ILLUMINA (Illumina HiSeq 2000) runs: 34.5M spots, 5G bases, 2.4Gb downloads

Design: RNA sequencing of beech roots and associated fungi

Submitted by: Forest Botany and Tree Physiology, University of Geottingen (Forest Botany and Tree Physiology, University of G)

Study: RNA sequencing of beech roots and associated fungi

PRJEB61297 • ERP146398 • All experiments • All runs

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Roots were collected from young trees outplanted in three regions in Germany (Schorfheide, Swabian Alb, Hainich) and used for RNA extraction. In each region four plots were sampled. Two plants used from each plot. The RNA of the plants per plot was pooled. Four technical replicates were prepared.

Sample: S1_S1

SAMEA112896190 • ERS14902329 • All experiments • All runs

Organism: Fagus sylvatica

Library:

Name: S1_S1_s

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Per plant: harvest of fine roots (< 2mm diameter) in the field, froozen in liquid nitrogen and transport to the lab on dry ice. Storage at -80°C until RNA extraction environmental samples Total RNA was extracted using the protocol of Chang S, Puryear J, Cairney J (1993, A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116). The RNA of four samples was pooled. The cDNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt am Main, Germany) according to the manufactorer´s protocol

Experiment attributes:

Experimental Factor: environmental history: Hainich

Runs: 4 runs, 34.5M spots, 5G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
ERR11218682	8,758,872	1.3G	630.4Mb	2023-04-23
ERR11218683	8,411,374	1.2G	608Mb	2023-04-23
ERR11218684	8,879,030	1.3G	637.5Mb	2023-04-23
ERR11218685	8,472,306	1.2G	610.5Mb	2023-04-23

ID:: 27467272

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